LOCUS       sequence-136638-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p306-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..222
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             223..1026
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 caccttttca attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat
       61 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
      121 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
      181 caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat
      241 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
      301 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
      361 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
      421 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
      481 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
      541 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
      601 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
      661 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
      721 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
      781 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
      841 gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca
      901 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
      961 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     1021 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     1081 atttaattat atcagttatt accctatgcg gtgtgaa
//
