LOCUS       sequence-136651-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-CNL
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    63..287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     276..293
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctgctaaagc gcatgctcca gactgccttg ggaaaagctg caggttcttt ccgcctcaga
       61 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      121 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      181 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      241 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      301 gcgagctcta gatcatcgat caagcttatc gagcggccgc tctagattac tgctcgttct
      361 tcagcactct ctccacgaag ctcttgatgt acttgcccat ctcgtcgggg gcgtcctcct
      421 ggaggaagtg caggcccttc accttcacga actcggtgtt ggggaacttc ttggcgccct
      481 ccacgatggc gttgctgaag aagccggggt cgccctcgat gaacagcttg ggcaggtcgt
      541 cgctggctct caggtaggcg ttgtagtttc tcacgatctg caccacgtcg ggcttgccgc
      601 ccttcaccag ggggatctct ctgggccagc tcagggtggg tcttctcacc tcgcccttct
      661 ccttgaaggg ctccaggtag gcggcgaact cctcgggctc cagctttctc atgatcttgc
      721 tgggcagcac ggtctccacg aagaagttgt tctccagcac catcttctcg ccctcctcgc
      781 tcttgatcag ggcgatgtcc tcctcgatgt ctggccactc gtcccagctc tcgatcacgt
      841 ccaccacgct ctccatgtgc acgatggcct tgatcctgtc ctggtgctcg taggcgtagt
      901 ggaaggccag ggcggcgccc cagtcgtggc ccacgaagat gatcttcttg ggcaggttca
      961 gaagctcgaa ccaggcggtc aggtacttgt a
//
