LOCUS       sequence-136652-         453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-CNL
FEATURES             Location/Qualifiers
     source          1..453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..43
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggggtac
       61 caaggtgtac gaccccgagc agaggaagag gatgatcacc ggcccccagt ggtgggccag
      121 gtgcaagcag atgaacgtgc tggacagctt catcaactac tacgacagcg agaagcacgc
      181 cgagaacgcc gtgatcttcc tgcacggcaa cgccactagc agctacctgt ggaggcacgt
      241 ggtgccccac atcgagcccg tggccaggtg catcatcccc gatctgatcg gcatgggcaa
      301 gagcggcaag agcggcaacg gcagctacag gctgctggac cactacaagt acctgaccgc
      361 ctggttcgag cttctgaacc tgcccaagaa gatcatcttc gtgggccacg actggggcgc
      421 cgccctggcc ttccactacg cctacgagca cca
//
