LOCUS       sequence-136660-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 induced gene disruption.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, 
            Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, 
            Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden 
            MG, Sabatini DM
  TITLE     SHMT2 drives glioma cell survival in ischaemia but imposes a 
            dependence on glycine clearance.
  JOURNAL   Nature. 2015 Apr 16;520(7547):363-7. doi: 10.1038/nature14363. Epub 
            2015 Apr 8.
  PUBMED    25855294
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14363"; journalName: "Nature"; date: "2015-04-16- 
            16"; volume: "520"; issue: "7547"; pages: "363-7"
COMMENT     Sequence Label: sgGLDC_2
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(313..333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(340..363)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      363..372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(392..603)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    672..789
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
       61 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
      121 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
      181 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
      241 gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg ctgctgggac
      301 tccgtggata ccgaccttcc gcttcttctt tggggccatc ttatcgtcat cgtctttgta
      361 atccatggtg gcggatcccg cgtcacgaca cctgtgttct ggcggcaaac ccgttgcgaa
      421 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
      481 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
      541 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
      601 cccactgacg ggcaccggag ccactcgagc cggcgccaaa gtggatctct gctgtccctg
      661 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
      721 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
      781 cttttaaaat tgtggatgaa tactgccatt tgtctcaaga tctagaattc aaaaaaagca
      841 ccgactcggt gccacttttt caagttgata acgg
//
