LOCUS       sequence-136661-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral backbone for cloning and expressing U6 driven sgRNAs with
            BfuAI cloning sites and puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.11.015"; journalName: "Cell"; date: "2015-12-3-
            3"; volume: "163"; issue: "6"; pages: "1515-26"
COMMENT     Sequence Label: pLCKO
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..254
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6..26
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     177..196
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
       61 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      121 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      181 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      241 aaggacgagg taccggtagg caggtgtgtt gtaaatgagc acacaaaata cacatgctaa
      301 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
      361 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
      421 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
      481 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
      541 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta
      601 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat
      661 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt
      721 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct
      781 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct
      841 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca
      901 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta
      961 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca
     1021 tcacataatt gtttaatt
//
