LOCUS       sequence-136662-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral backbone for cloning and expressing U6 driven sgRNAs with
            BfuAI cloning sites and puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.11.015"; journalName: "Cell"; date: "2015-12-3-
            3"; volume: "163"; issue: "6"; pages: "1515-26"
COMMENT     Sequence Label: pLCKO
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    140..257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(305..380)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
       61 gctgccttgg aaaaggcgca acccgggccc cctcgagccg gcgccaaagt ggatctctgc
      121 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
      181 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
      241 ccccttttct tttaaaattg tggatgaata ctgccatttg tctcaagatc tagaattcaa
      301 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
      361 gctatttcta gctctaaaac ctctgcaggt ctacaaaaaa gagcaagaag ctaaaaaaga
      421 tttaaaaatt atttttagcg cagttaatgg aacaggaact aaatttaccc caaaaatatt
      481 acgtgaatca ggatataacg ttattgaggt tgaagagcat gcatttgaag atgaaacatt
      541 taaaaatgtt gtaaatccaa atccagaatt tgatcctgca tgaaaaatac cgcttgaata
      601 tggtattaaa catgatgcag atattattat tatgaatgac ccagatgctg acagatttgg
      661 aatggcaata aaacatgatg gtcattttgt aagattagat ggaaatcaaa caggaccaat
      721 tttaattgat tgaaaattat caaatctaaa acgcttaaat agcattccaa aaaatccggc
      781 tctatattca agttttgtaa caagtgattt gggtgataga atcgctcatg aaaaatatgg
      841 agttaatatt gtaaaaactt taactggatt taaatgaatg ggtagagaaa ttgctaaaga
      901 agaagataac ggattaaatt ttgtttttgc ttatgaagaa agttatggat atgtaattga
      961 tgactcagct agagataaag atggaataca agcttctata ttaatagcag aggctgcttg
     1021 attttataaa aa
//
