LOCUS       sequence-136664-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1055
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..117)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(124..144)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttagcagccg gatcagcgcg cacgcgtggt accggccggc caccaggtgg cgcgccgcga
       61 tcgcggatcc gttatccact tccaatgtta ttaatcaact tttcgtttct ttttaggatc
      121 ctcaactttt cgttttttct taggtgaccc gtcacctcct agctgactca aatcaatgcg
      181 tgtttcataa agaccagtga tggattgatg gataagagtg gcatctaaaa cttcttttgt
      241 agacgtatat cgtttacgat caattgttgt atcaaaatat ttaaaagcag cgggagctcc
      301 aagattcgtc aacgtaaata aatgaataat attttctgct tgttcacgta ttggtttgtc
      361 tctatgtttg ttatatgcac taagaacttt atctaaattg gcatctgcta aaataacacg
      421 cttagaaaat tcactgattt gctcaataat ctcatctaaa taatgcttat gctgctccac
      481 aaacaattgt ttttgttcgt tatcttctgg actacccttc aacttttcat aatgactagc
      541 taaatataaa aaattcacat atttgcttgg cagagccagc tcatttcctt tttgtaattc
      601 tccggcacta gccagcatcc gtttacgacc gttttctaac tcaaaaagac tatatttagg
      661 tagtttaatg attaagtctt ttttaacttc cttatatcct ttagcttcta aaaagtcaat
      721 cggatttttt tcaaaggaac ttctttccat aattgtgatc cctagtaact ctttaacgga
      781 ttttaacttc ttcgatttcc ctttttccac cttagcaacc actaggactg aataagctac
      841 cgttggacta tcaaa
//
