LOCUS       sequence-136666-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1055
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             49..71
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt
       61 taagaaggag atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg
      121 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
      181 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
      241 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
      301 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
      361 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
      421 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
      481 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
      541 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
      601 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
      661 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
      721 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
      781 gaaacagcga tgaccatcaa cggcccgtg
//
