LOCUS       sequence-136678-        1908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hRPN13 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-HIS-TEV-GST-PP-RPN13
FEATURES             Location/Qualifiers
     source          1..1908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..684
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggaagttc tgttccaggg gccaatgacg acctcaggcg cgctctttcc aagcctggtg
      721 ccaggctctc ggggcgcctc caacaagtac ttggtggagt ttcgggcggg aaagatgtcc
      781 ctgaagggga ccaccgtgac tccggataag cggaaagggc tggtgtacat tcagcagacg
      841 gacgactcgc ttattcactt ctgctggaag gacaggacgt ccgggaacgt ggaagacgac
      901 ttgatcatct tccctgacga ctgtgagttc aagcgggtgc cgcagtgccc cagcgggagg
      961 gtctacgtgc tgaagttcaa ggcagggtcc aagcggcttt tcttctggat gcaggaaccc
     1021 aagacagacc aggatgagga gcattgccgg aaagtcaacg agtatctgaa caaccccccg
     1081 atgcctgggg cactgggggc cagcggaagc agcggccacg aactctctgc gctaggcggt
     1141 gagggtggcc tgcagagcct gctgggaaac atgagccaca gccagctcat gcagctcatc
     1201 ggaccagccg gcctcggagg actgggtggg ctgggggccc tgactggacc tggcctggcc
     1261 agtttactgg ggagcagtgg gcctccaggg agcagctcct cctccagctc ccggagccag
     1321 tcggcagcgg tcaccccgtc atccaccacc tcttccaccc gtgccacccc agccccttct
     1381 gctccagcag ctgcctcagc aactagcccg agccccgcgc ccagttccgg gaatggagcc
     1441 agcacagcag ccagcccgac ccagcccatc cagctgagcg acctccagag catcctggcc
     1501 acgatgaacg taccagccgg gccagcaggc ggccagcaag tggacctggc cagtgtgctg
     1561 acgccggaga taatggctcc catcctcgcc aacgcggatg tccaggagcg cctgcttccc
     1621 tacttgccat ctggggagtc gctgccgcag accgcggatg agatccagaa taccctgacc
     1681 tcgccccagt tccagcaggc cctgggcatg ttcagcgcag ccttggcctc ggggcagctg
     1741 ggccccctca tgtgccagtt cggtctgcct gcagaggctg tggaggccgc caacaagggc
     1801 gatgtggaag cgtttgccaa agccatgcag aacaacgcca agcccgagca gaaagagggc
     1861 gacacgaagg acaagaagga cgaagaggag gacatgagcc tggactga
//
