LOCUS       sequence-136679-        9250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent FPX sensor for cAMP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: pPHT-PKA
FEATURES             Location/Qualifiers
     source          1..9250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2657..2710
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(4675..4692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4681..4905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4951..5379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5038..5057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5248..5269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5388..5408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5393..5722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5573..5708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5635..5654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5771..6796
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5771..6796
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6926..7047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6963..6982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7017..7036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7096..7112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7096..7112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7109..7131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7120..7136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7144..7150,7151..7168,7169..7174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7189..7210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7327..7344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7498..8083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7578..7597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8254..9045,9046..9114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8877..8896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9115..9219)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaccatg gtgagcaaga gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
      961 cttggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     1081 ctgggacatc ctgtcccccc agatcatgta cggctccaag gcgtacgtga agcaccccgc
     1141 cgacgtcccc gattacatga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1201 gcacttcgag gacggcggtc tggtgaccgt gactcaggac acctccctgc aggacggcac
     1261 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     1321 gaagaagacc ttgggctggg attatgccac cgagcgcctg taccccgaag acggcgtgct
     1381 gaagggcgag cttctggggc gcctgaagct gaaggacggc ggcctcaacc tggtggagtt
     1441 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact tcgtggacac
     1501 caagctgggc atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     1561 cgagggccgc caccacctgg gcatggacga gctgtacaag ggtggaggca acgccgccgc
     1621 cgccaagaag ggcagcgagc aggagagcgt gaaagagttc ctagccaaag ccaaggaaga
     1681 tttcctgaaa aaatgggaga ccccttctca gaatacagcc cagttggatc agtttgatag
     1741 aatcaagacc cttggcaccg gctcctttgg gcgagtgatg ctggtgaagc acaaggagag
     1801 tgggaaccac tacgccatga agatcttaga caagcagaag gtggtgaagc taaagcagat
     1861 cgagcacact ctgaatgaga agcgcatcct gcaggccgtc aacttcccgt tcctggtcaa
     1921 acttgaattc tccttcaagg acaactcaaa cctgtacatg gtcatggagt atgtagctgg
     1981 tggcgagatg ttctcccacc tacggcggat tggaaggttc agcgagcccc atgcccgttt
     2041 ctacgcggcg cagatcgtcc tgacctttga gtatctgcac tccctggacc tcatctaccg
     2101 ggacctgaag cccgagaatc ttctcatcga ccagcagggc tatattcagg tgacagactt
     2161 cggttttgcc aagcgtgtga aaggccgtac ttggaccttg tgtgggaccc ctgagtactt
     2221 ggcccccgag attatcctga gcaaaggcta caacaaggct gtggactggt gggctctcgg
     2281 agtcctcatc tacgagatgg ctgctggtta cccacccttc ttcgctgacc agcctatcca
     2341 gatctatgag aaaatcgtct ctgggaaggt gcggttccca tcccacttca gctctgactt
     2401 gaaggacctg ctgcggaacc ttctgcaggt ggatctcacc aagcgctttg ggaacctcaa
     2461 gaacggggtc aatgacatca agaaccacaa gtggtttgcc acgactgact ggattgccat
     2521 ctatcagaga aaggtggaag ctcccttcat accaaagttt aaaggccctg gggacacgag
     2581 taactttgac gactatgagg aggaagagat ccgggtctcc atcaatgaga agtgtggcaa
     2641 ggagtttact gagtttgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     2701 tcccgggcct agcatcgaga tcccggcggg actgacggag ctgctgcagg gcttcacggt
     2761 ggaggtgctg aggcaccagc ccgcggacct gctggagttc gctctgcagc acttcacccg
     2821 cctgcagcag gagaacgagc gcaaaggcac cgcgcgcttc tgccatgagg gcaggacctg
     2881 gggggacctg ggcgccgctg ccgggggcgg cacccccagc aagggggtca acttcgccga
     2941 ggagcccatg cagtccgact ccgaggacgg ggaggaggag gaggcggcgc ccgcggacgc
     3001 aggggcgttc aatgctccag taataaaccg attcacaagg cgtgcctcag tatgtgcaga
     3061 agcttataat cctgatgaag aagaagatga tgcagagtcc aggattatac atccaaaaac
     3121 tgatgatcaa agaaataggt tgcaagaggc ttgcaaagac atcctgctgt ttaagaatct
     3181 ggatccggag cagatgtctc aagtattaga tgccatgttt gaaaaattgg tcaaagatgg
     3241 ggagcatgta attgatcaag gtgacgatgg tgacaacttt tatgtaattg atagaggcac
     3301 atttgatatt tatgtgaaat gtgatggtgt tggaagatgt gttggtaact atgataatcg
     3361 tgggagtttc ggcgaactgg ccttaatgta caatacaccc agagcagcta caatcactgc
     3421 tacctctcct ggtgctctgt ggggtttgga cagggtaacc ttcaggagaa taattgtgaa
     3481 aaacaatgcc aaaaagagaa aaatgtatga aagctttatt gagtcactgc cattccttaa
     3541 atctttggag ttttctgaac gcctgaaagt agtagatgtg ataggcacca aagtatacaa
     3601 cgatggagaa caaatcattg ctcagggaga ttcggctgat tcttttttca ttgtagaatc
     3661 tggagaagtg aaaattacta tgaaaagaaa gggtaaatca gaagtggaag agaatggtgc
     3721 agtagaaatc gctcgatgct cgcggggaca gtactttgga gagcttgccc tggtaactaa
     3781 caaacctcga gcagcttctg cccacgccat tgggactgtc aaatgtttag caatggatgt
     3841 gcaagcattt gaaaggcttc tgggaccttg catggaaatt atgaaaagga acatcgctac
     3901 ctatgaagaa cagttagttg ccctgtttgg aacgaacatg gatattgttg aacccactgc
     3961 aggtggaggc ggtggagtga gcaagggcga ggagaccatc aaagagttca tgcgcttcaa
     4021 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4081 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4141 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccgaggcgt acgtgaggca
     4201 ccccgccgac atccccgatt acaagaagct gcccttcccc gagggcttca agtgggagcg
     4261 cgtgatgaac ttcgaagacg gcggtctggt gaccgttacc caggactcct ccctgcagga
     4321 cggcacgctg atctgcaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     4381 aatgcagaag aagaccatgg gctgggaggc ctccaccgag atgctgtacc ccgaagacgg
     4441 cgtgctgaag ggccatagct atcaggccct gaagctgaag gacggcggcc actacctggt
     4501 ggagttcgag accatctaca tggccaagaa gcccgtgcaa ctgcccggcg attactgtgt
     4561 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacgg
     4621 gcgctccgag ggccgccacc gtctgggcat ggacgagcgg tacaagtagc gcgccctcga
     4681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4921 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5341 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5401 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5461 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5521 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5581 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5641 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5701 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5761 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     5821 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     5881 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     5941 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     6001 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     6061 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     6121 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     6181 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     6241 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     6301 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     6361 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     6421 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     6481 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     6541 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     6601 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     6661 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     6721 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     6781 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     6841 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6901 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7021 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7621 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7681 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7741 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7801 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7861 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7921 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7981 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     8041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9241 acctgacgtc
//
