LOCUS       sequence-136681-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3X Flag with P2A-Neo for 3' tagging of endogenous proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_Donor (EMM0021)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..110)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(126..149)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(180..203)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccaggtcca
       61 gggttctcct ccacgtctcc agcctgcttc agcaggctga agttagtagc tccgcttcct
      121 gaaaccttat cgtcgtcatc cttgtaatct cccttatcgt cgtcatcctt gtaatcgccc
      181 ttatcgtcgt catccttgta atctccagaa ccacctccac cggaacctcc tccgctcaga
      241 gacccgcgta aggtctcaag ctgggctgca ggtcggggag aggagtgtta gtaaccgcga
      301 cgctggtggg ggtcggcttg ttaagagggg cgctgctaac gctgcaagag tgggttgtca
      361 gcgtggggcc ggtgctactg gaatcgatac cggcatgatt gacagcctgg gcgaggatgt
      421 cacctgatgg tgataagaat acacgggaga cttagtacgg tttcacaggc gtgacacgtt
      481 tattgagtag gattacagag tataacatag agtataatat agagtataca atagtgacgt
      541 gggatcctca gttagcctcc cccatctccc gggcaaacgt gcgcgccagg tcgcagatcg
      601 tcggtatgga gccgggggtg gtgacgtggg tctggaccat cccggaggta agttgcagca
      661 gggcgtcccg gcagccggcg ggcgattggt cgtaatccag gataaagacg tgcatgggac
      721 ggaggcgttt ggccaagacg tccaaggccc aggcaaacac gttgtacagg tcgccgttgg
      781 gggccagcaa ctcgggggcc cgaaacaggg taaataacgt gtccccgata tggggtcgtg
      841 ggcccgcgtt gctctggggc tcggcaccct gggg
//
