LOCUS       sequence-136690-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Synechocystis PCC6803 orf sll1489 (synthetic gene).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Kappock
  TITLE     Synechocystis PCC6803 CpmA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK681
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcaagcg
       61 gcgtggatct gggcaccgaa aatctgtact ttcagtctgt gtctggcccg gtcttccagt
      121 ttagtgcacc gatgacgccg gatagcctgc aacagctgct gacggcaatc gcaagcggtc
      181 acattagctc tcaagaaggc tttgaacagc tgaaacatct gagttttcag gcgatcgatg
      241 acttcgccaa agtggatcac cagcgtcaac tgcgcaccgg tttcccggaa gtggtttggg
      301 gcccgggtaa aacgccggaa cagattgaac aaattatcca ggtcctggca gtgcataacc
      361 cggtcgtgct ggttacccgt gtcgaaccgg aagtggctga tctgctgggc gaccgtattc
      421 cgctgctgca atattacccg caggcacgca tctgcgctct ggttcaaacc ccgctgcaga
      481 ttaaatattc aggcaccatc ggtgttctgt cggccggtac ggcagatctg ccggtcgcag
      541 aagaagcggc cattacggcc agcctgtgtg gttttaaagt tgaaaaactg tgggatgttg
      601 gcgtcgcagg gatccatcgt ctgctgtctc accgcgaact gattcaggcc atggacgtgc
      661 tgatcgttgt cgcaggcatg gaaggtgctc tgccgtccgt ggttgcaggc ctggttgatt
      721 gcccggtgat cggtgttccg accagtgtcg gctacggcac gtcctttggc ggtgtggcac
      781 cgctgctgac catgctgaat agctgtgctg tgggcgttgg tgtcgtgaac attgacaatg
      841 gcttcggtgc agctatgctg gccggtcaga tcctgcgtac cgttgaccgt ctgcaaagta
      901 aatccac
//
