LOCUS       sequence-136692-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK553
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             688..705
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(758..776)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      772..819
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgat gagcgaaacc gcccccctgc
      121 cctctgcttc cagtgcttta gaagataaag cagcctctgc ccccgttgtg ggcattatca
      181 tgggcagcca gtcagactgg gaaaccatgc gccatgcaga cgcattgctg acagagctgg
      241 aaattccgca tgaaaccctc attgtttcag cacatcgtac gccagaccgg ttggcggatt
      301 atgcgcgtac ggcagccgaa cgcggcctga atgtgattat tgctggggcc ggtggagctg
      361 cacatctgcc gggtatgtgt gcggcatgga cacgcctgcc cgtgctgggt gttccagtag
      421 aatctcgcgc tcttaaaggc atggacagcc tgctttccat tgtgcagatg cccggcggcg
      481 tgcctgtggg cactttggct attggggcat ccggcgccaa aaatgccgcc ttgctggccg
      541 cctccattct tgcactgtat aacccggccc ttgccgcacg ccttgaaaca tggcgcgcat
      601 tgcagacggc ctctgtcccc aactctccca ttaccgaaga taaatgagaa ttcgagctcc
      661 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      721 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      781 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      841 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      901 c
//
