LOCUS       sequence-136693-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK553
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(648..665)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(675..692)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             711..733
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    748..772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(772..791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(773..791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(875..894)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tctcatttat cttcggtaat gggagagttg
      121 gggacagagg ccgtctgcaa tgcgcgccat gtttcaaggc gtgcggcaag ggccgggtta
      181 tacagtgcaa gaatggaggc ggccagcaag gcggcatttt tggcgccgga tgccccaata
      241 gccaaagtgc ccacaggcac gccgccgggc atctgcacaa tggaaagcag gctgtccatg
      301 cctttaagag cgcgagattc tactggaaca cccagcacgg gcaggcgtgt ccatgccgca
      361 cacatacccg gcagatgtgc agctccaccg gccccagcaa taatcacatt caggccgcgt
      421 tcggctgccg tacgcgcata atccgccaac cggtctggcg tacgatgtgc tgaaacaatg
      481 agggtttcat gcggaatttc cagctctgtc agcaatgcgt ctgcatggcg catggtttcc
      541 cagtctgact ggctgcccat gataatgccc acaacggggg cagaggctgc tttatcttct
      601 aaagcactgg aagcagaggg caggggggcg gtttcgctca tcatatggct gccgcgcggc
      661 accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt
      721 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      781 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc
      841 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      901 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      961 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcac
//
