LOCUS       sequence-136694-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); 
            N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe 
            mutant (AaPurK-V319F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK552
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             73..81
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
       61 gatatacata tgacctcctt acattctccg cttaagccca atgccgttat tggcattgtg
      121 gggggtggcc agcttggccg catgtctgct gttgctgccg cgcgccttgg ttttcgcacg
      181 catattctta cgccagaggc caacggcccg gcggctcagg tctctcacgc cgtaacatgc
      241 ggcaagtatg atgatccgac agcactggat gcctttgccc gcgctgttga tgttgttacc
      301 tttgaatttg aaaacatcag cgcagatgca ctggaccgcc tagccaaaca ctgccctgtt
      361 catccttcgg ggcatattct gcgcatcagt caggaccgat tggcagaaaa aacttttttt
      421 gcttctgcgg gtattccact cgcgccatgg catcctgtta ccgatgtcaa aacgctacac
      481 gaagccgcag aaaaagtggg tttgccgctg attctcaaaa ccactcgcaa tggatatgat
      541 ggcaaaggcc agcgccgtgt gcatcacatc catgatctgg aagaggcttt caacgctctg
      601 gcaccacatc ctctggttgc agaaggtatg gtggattttg cttgcgagat tagtgttatg
      661 gtggcgcgtg gtgcagatgg cacggtaagg tgctttgacc cggcgctgaa ccggcactgg
      721 aacggtattc tggatctaac attagccccc gcccccattg acccagatat tgccacgcag
      781 gccataaaac tagccaccac cattgcagaa aagctggagc tggttggtat tctgggcgtt
      841 gaaatgtttg ttgattgcac cggcaacctg ctggtgaatg aaatggcccc gcgcccccat
      901 aattctggcc attggaccat gaatgcctgc ccgcaggacc aatttgacat gcatatccgt
      961 gcagttgcgg ggctgcc
//
