LOCUS       sequence-136695-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); 
            N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe 
            mutant (AaPurK-V319F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK552
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..48)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac cagactcgag
       61 tgcggccgca agcttgtcga cggagctcga attcttaact ggcagattct tcatcttcca
      121 cagcggccgt tttggtggcc aatgggccca aggcatcctg aatacccaaa ttgcctggca
      181 gcgcaccgta ggggaacaga atatttacat gccccatttt acggcccact ctggcttctg
      241 ccttgccata tagatgcgga ataaagccct gcgtggccat aatctgcggc agcagcgcca
      301 tatcttccgg cccgaacaga tttttcatga ttgcatcaga atggcgcaca gcgtgcggca
      361 aaggcagccc cgcaactgca cggatatgca tgtcaaattg gtcctgcggg caggcattca
      421 tggtccaatg gccagaatta tgggggcgcg gggccatttc attcaccagc aggttgccgg
      481 tgcaatcaac aaacatttca acgcccagaa taccaaccag ctccagcttt tctgcaatgg
      541 tggtggctag ttttatggcc tgcgtggcaa tatctgggtc aatgggggcg ggggctaatg
      601 ttagatccag aataccgttc cagtgccggt tcagcgccgg gtcaaagcac cttaccgtgc
      661 catctgcacc acgcgccacc ataacactaa tctcgcaagc aaaatccacc ataccttctg
      721 caaccagagg atgtggtgcc agagcgttga aagcctcttc cagatcatgg atgtgatgca
      781 cacggcgctg gcctttgcca tcatatccat tgcgagtggt tttgagaatc agcggcaaac
      841 ccactttttc tgcggcttcg tgtagcgttt tgacatcggt aacaggatgc catggcgcga
      901 gtggaatacc cgcagaagca aaaaaagttt tttctgccaa tcggtcctga ctgatgcgca
      961 gaatatgccc cgaaggatga acagggcagt gtttggctag gcggtccagt gcatctgcgc
     1021 tgatgttttc aaatt
//
