LOCUS       sequence-136700-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein with 
            N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole 
            ribonucleotide mutase (AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK538
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgctgcga gatcgcgatt
      121 tactgcttgc ccaactgcat gaactccgga gtgaacacag agatctggat acggttatca
      181 accgcatggc gcatgaaact acttttattg accaactcta tctccagcgg ctgaaaaaac
      241 gtaaacttct gctaaaagat cagattacca aggttgaaag ccttctgatc cctgatgaca
      301 ttgcctgaac ctctccgtta cagactttaa aaaaccggat agatacccac cgtgagcgaa
      361 accgcccccc tgccctctgc ttccagtgct ttagaagata aagcagcctc tgcccccgtt
      421 gtgggcatta tcatgggcag ccagtcagac tgggaaacca tgcgccatgc agacgcattg
      481 ctgacagagc tggaaattcc gcatgaaacc ctcattgttt cagcacatcg tacgccagac
      541 cggttggcgg attatgcgcg tacggcagcc gaacgcggcc tgaatgtgat tattgctggg
      601 gccggtggag ctgcacatct gccgggtatg tgtgcggcat ggacacgcct gcccgtgctg
      661 ggtgttccag tagaatctcg cgctcttaaa ggcatggaca gcctgctttc cattgtgcag
      721 atgcccggcg gcgtgcctgt gggcactttg gctattgggg catccggcgc caaaaatgcc
      781 gccttgctgg ccgcctccat tcttgcactg tataacccgg cccttgccgc acgccttgaa
      841 acatggcgcg cattgcagac ggcctctgtc cccaactctc
//
