LOCUS       sequence-136701-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein with 
            N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole 
            ribonucleotide mutase (AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK538
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(891..908)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(918..935)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             954..976
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattctc atttatcttc ggtaatggga gagttgggga
      121 cagaggccgt ctgcaatgcg cgccatgttt caaggcgtgc ggcaagggcc gggttataca
      181 gtgcaagaat ggaggcggcc agcaaggcgg catttttggc gccggatgcc ccaatagcca
      241 aagtgcccac aggcacgccg ccgggcatct gcacaatgga aagcaggctg tccatgcctt
      301 taagagcgcg agattctact ggaacaccca gcacgggcag gcgtgtccat gccgcacaca
      361 tacccggcag atgtgcagct ccaccggccc cagcaataat cacattcagg ccgcgttcgg
      421 ctgccgtacg cgcataatcc gccaaccggt ctggcgtacg atgtgctgaa acaatgaggg
      481 tttcatgcgg aatttccagc tctgtcagca atgcgtctgc atggcgcatg gtttcccagt
      541 ctgactggct gcccatgata atgcccacaa cgggggcaga ggctgcttta tcttctaaag
      601 cactggaagc agagggcagg ggggcggttt cgctcacggt gggtatctat ccggtttttt
      661 aaagtctgta acggagaggt tcaggcaatg tcatcaggga tcagaaggct ttcaaccttg
      721 gtaatctgat cttttagcag aagtttacgt tttttcagcc gctggagata gagttggtca
      781 ataaaagtag tttcatgcgc catgcggttg ataaccgtat ccagatctct gtgttcactc
      841 cggagttcat gcagttgggc aagcagtaaa tcgcgatctc gcagcatatg gctgccgcgc
      901 ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt atatctcctt
      961 cttaaagtta aacaaaatta tttctagagg ggaattgtt
//
