LOCUS       sequence-136702-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate 
            synthetase (AaPrsAH6-insA2), C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK631
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             978..995
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1048..1066)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggc taaaatcgtc gcctgtaaca
       61 gcaacctccc acttgccgaa gccgttgccg cggagctgaa cctccccctc tgcaacgcca
      121 ccgtgcgccg ctttgcggac atggaggtgt ttgtagaaat ccacgaaaat gtacgtggtg
      181 aggacgtgtt cgttatccag agcacctgca cccccaccaa tgataacctg atggaactgc
      241 tgattatgct ggatgcgctg cgccgtggtt cggccaagcg tattacagcc gttatgccct
      301 attttgggta tgcccggcag gatcgtaaat ctggcccgcg cacgcccatt agcgccaaac
      361 tggtggcgaa tctgttggtg gaagcgggcg ccagccgcgt gctgacactg gacctgcacg
      421 ccatgcagat tcagggcttt tttgatattc cggtggacaa tctgtacgcc gcgcccttgt
      481 ttgtgcgcga tatccgctcc cattacgggg accgcgattt aatgattgtc tcccccgatg
      541 taggtggtgt ggtgcgtgcc cgccagctgg cccagcgcct gaacacggat ctggccatta
      601 ttgacaagcg gcgtgaacgc gcaggcgttt ccgaagtgat gaacgtgatt ggtgatgtgc
      661 gcggccgcca ctgcctgctg gtagatgata ttgtggatag cggtggttca ctgtgcaatg
      721 cagcccgcgc cattgccgag cagggtgccg catctgtggg cgcatacgta acgcatggtg
      781 tgctgacagg acacgccgtg gaacgcattg ccgattcgcc tatcgaaatg ctgacgctga
      841 cagacagcat tcgcgctaca gagcaggtta tggccgcacg caatatccgc cagatcacta
      901 tttctggcct gctggcgcgc gccatccatg ccgtggcgga tgaaagctcc gtttcctccc
      961 tgtttgactc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     1021 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     1081 cctctaa
//
