LOCUS       sequence-136703-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate 
            synthetase (AaPrsAH6-insA2), C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK631
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1001..1023
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1054..1073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1055..1073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 agtcaaacag ggaggaaacg gagctttcat ccgccacggc atggatggcg cgcgccagca
      121 ggccagaaat agtgatctgg cggatattgc gtgcggccat aacctgctct gtagcgcgaa
      181 tgctgtctgt cagcgtcagc atttcgatag gcgaatcggc aatgcgttcc acggcgtgtc
      241 ctgtcagcac accatgcgtt acgtatgcgc ccacagatgc ggcaccctgc tcggcaatgg
      301 cgcgggctgc attgcacagt gaaccaccgc tatccacaat atcatctacc agcaggcagt
      361 ggcggccgcg cacatcacca atcacgttca tcacttcgga aacgcctgcg cgttcacgcc
      421 gcttgtcaat aatggccaga tccgtgttca ggcgctgggc cagctggcgg gcacgcacca
      481 caccacctac atcgggggag acaatcatta aatcgcggtc cccgtaatgg gagcggatat
      541 cgcgcacaaa caagggcgcg gcgtacagat tgtccaccgg aatatcaaaa aagccctgaa
      601 tctgcatggc gtgcaggtcc agtgtcagca cgcggctggc gcccgcttcc accaacagat
      661 tcgccaccag tttggcgcta atgggcgtgc gcgggccaga tttacgatcc tgccgggcat
      721 acccaaaata gggcataacg gctgtaatac gcttggccga accacggcgc agcgcatcca
      781 gcataatcag cagttccatc aggttatcat tggtgggggt gcaggtgctc tggataacga
      841 acacgtcctc accacgtaca ttttcgtgga tttctacaaa cacctccatg tccgcaaagc
      901 ggcgcacggt ggcgttgcag agggggaggt tcagctccgc ggcaacggct tcggcaagtg
      961 ggaggttgct gttacaggcg acgattttag ccatggtata tctccttctt aaagttaaac
     1021 aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc
     1081 gggatc
//
