LOCUS       sequence-136705-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK550
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..18
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             131..175
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(213..231)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      227..274
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag aaggagatat
       61 acatatggca gatctcaatt ggatatcggc cggccacgcg atcgctgacg tcggtaccct
      121 cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg actcgtctac
      181 tagcgcagct taattaacct aggctgctgc caccgctgag caataactag cataacccct
      241 tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca
      301 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
      361 tatttaacga ccctgccctg aaccgacgac cgggtcatcg tggccggatc ttgcggcccc
      421 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
      481 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
      541 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
      601 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
      661 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
      721 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
      781 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
      841 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
      901 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtc
//
