LOCUS       sequence-136706-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK550
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    155..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(180..198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    884..908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(909..927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgaggg
       61 taccgacgtc agcgatcgcg tggccggccg atatccaatt gagatctgcc atatgtatat
      121 ctccttctta tacttaacta atatactaag atggggaatt gttatccgct cacaattccc
      181 ctatagtgag tcgtattaat ttcgattatg cggccgtgta caatacgatt actttctgtt
      241 cgacttaagc attatgcggc cgcaagcttg tcgacggagc tcgaattctc atttatcttc
      301 ggtaatggga gagttgggga cagaggccgt ctgcaatgcg cgccatgttt caaggcgtgc
      361 ggcaagggcc gggttataca gtgcaagaat ggaggcggcc agcaaggcgg catttttggc
      421 gccggatgcc ccaatagcca aagtgcccac aggcacgccg ccgggcatct gcacaatgga
      481 aagcaggctg tccatgcctt taagagcgcg agattctact ggaacaccca gcacgggcag
      541 gcgtgtccat gccgcacaca tacccggcag atgtgcagct ccaccggccc cagcaataat
      601 cacattcagg ccgcgttcgg ctgccgtacg cgcataatcc gccaaccggt ctggcgtacg
      661 atgtgctgaa acaatgaggg tttcatgcgg aatttccagc tctgtcagca atgcgtctgc
      721 atggcgcatg gtttcccagt ctgactggct gcccatgata atgcccacaa cgggggcaga
      781 ggctgcttta tcttctaaag cactggaagc agagggcagg ggggcggttt cgctcaccat
      841 ggtatatctc cttattaaag ttaaacaaaa ttatttctac aggggaattg ttatccgctc
      901 acaattcccc tatagtgagt cgtattaatt tcctaatgca ggagtcgcat aagggagagc
      961 gtcgagatcc cggacaccat cgaatgg
//
