LOCUS       sequence-136708-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK547
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gtgcggccgc aagcttgtcg acggagctcg aattcttaac tggcagattc ttcatcttcc
      121 acagcggccg ttttggtggc caatgggccc aaggcatcct gaatacccaa attgcctggc
      181 agcgcaccgt aggggaacag aatatttaca tgccccattt tacggcccac tctggcttct
      241 gccttgccat atagatgcgg aataaagccc tgcgtggcca taatctgcgg cagcagcgcc
      301 atatcttccg gcccgaacag atttttcatg attgcatcag aatggcgcac agcgtgcggc
      361 aaaggcagcc ccgcaactgc acggatatgc atgtcaaatt ggtcctgcgg gcaggcattc
      421 atggtccaat ggccagaatt atgggggcgc ggggccattt cattcaccag caggttgccg
      481 gtgcaatcaa caaacatttc aacgcccaga ataccaacca gctccagctt ttctgcaatg
      541 gtggtggcta gttttatggc ctgcgtggca atatctgggt caatgggggc gggggctaat
      601 gttagatcca gaataccgtt ccagtgccgg ttcagcgccg ggtcaaagca ccttaccgtg
      661 ccatctgcac cacgcgccac cataacacta atctcgcaag caaaatccac cataccttct
      721 gcaaccagag gatgtggtgc cagagcgttg aaagcctctt ccagatcatg gatgtgatgc
      781 acacggcgct ggcctttgcc atcatatcca ttgcgagtgg ttttgagaat cagcggcaaa
      841 cccacttttt ctgcggcttc gtgtagcgtt ttgacatcgg taacaggatg ccatggcgcg
      901 agtggaatac ccgcagaagc aaaaaaagtt ttttctgcca atcggtcctg actgatgcgc
      961 agaatatgcc ccgaaggatg aacagggcag tgtttggcta ggcggtccag tgcatctgcg
     1021 ctga
//
