LOCUS       sequence-136720-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal 
            His6 tag (H6EcGrxD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK690
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(448..465)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(475..492)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             511..533
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    548..572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(572..591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(573..591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..694)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc ggatccttat tccgcgtccg gctcttcaga
      121 cttgtattta gcggcagttt ctttgatcag ctgctgcagt tcgccacgct gatacatttc
      181 gatcacgata tcacaaccgc cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg
      241 ccagttagca tatttcggca gttccgcacg aatgtccgga ttctgcagaa tatcaacata
      301 ggcaaaacgt tcgccacatg cggcaagcgc ctggactgcc tgggcagaga aaccgcagct
      361 cggcagtttc ggtgaacctt tcatgtacag caggatcggg ttttcagcaa tctggcgttg
      421 gattttttcg atagtggtgc tcatatggct gccgcgcggc accaggccgc tgctgtgatg
      481 atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt
      541 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      601 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      661 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      721 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      781 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      841 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
      901 acaccatcga atggcgcaaa acctttc
//
