LOCUS       sequence-136726-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes (AaOrfX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK536
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             264..281
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(334..352)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      348..395
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      432..887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(519..538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     729..750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc tgcgtgatcg cgacctgctg
       61 ctggcgcaat tgcatgaact gcgtagcgaa caccgcgatc tggacaccgt gatcaaccgt
      121 atggcccatg aaaccacgtt tatcgatcag ctgtatctgc aacgtctgaa aaaacgcaaa
      181 ctgctgctta aggatcaaat tacgaaagtg gaaagtctac tgattccgga tgacatcgcg
      241 tgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      301 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      361 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      421 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      841 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      961 ccgctcatga gacaataacc ctgataaatg cttc
//
