LOCUS       sequence-136727-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 small hypothetical protein adjacent 
            to purEK genes (AaOrfX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK536
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             282..304
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(335..354)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(336..354)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttca cgcgatgtca tccggaatca gtagactttc cactttcgta atttgatcct
      121 taagcagcag tttgcgtttt ttcagacgtt gcagatacag ctgatcgata aacgtggttt
      181 catgggccat acggttgatc acggtgtcca gatcgcggtg ttcgctacgc agttcatgca
      241 attgcgccag cagcaggtcg cgatcacgca gcatatgtat atctccttct taaagttaaa
      301 caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt attaatttcg
      361 cgggatcgag atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
      421 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
      481 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
      541 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
      601 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
      661 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
      721 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
      781 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
      841 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
      901 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
      961 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     1021 cgcgcg
//
