LOCUS       sequence-136730-         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola ATCC 35405 PurE with a TEV 
            protease-cleavable N-terminal His6 tag (H6tTdPurE2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK636
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcgagctccg tcgacaagaa actgaaacgc tgaattcagc gtttcagttt
      121 cgaatcgtct tcgataattt tctgcgcatt ggattccata taggatttga ccgaatctgc
      181 gatttcttta tcgtacagtg aaaaaatacg agccgccagg agtgccgcat ttttcggttc
      241 aagaaccagt gccggactaa tcccgctcgg catccgtaac gaagaataaa tgtcggcgcc
      301 ggcgaagcta tcgcttggcg gtgggcaggc aatggttgca cctttgacga agccatcaac
      361 aaagcctgat aaagcgttac tacgcccagc gatcgtgatg tacagtttag gacggtccaa
      421 agcttcatat tctttcagca tagacactac atgctccgct gttttatgcg cggagccgat
      481 gcgaatggca tactcaatac caaaag
//
