LOCUS       sequence-136735-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Large T Antigen. Used to create 
            immortalized cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pBABE-neo largeTcDNA
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             404..424
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
       61 gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc ctctgatgag
      121 aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa
      181 aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat atgctcatca
      241 acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa ctgatgaatg
      301 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
      361 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
      421 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
      481 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
      541 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
      601 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
      661 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
      721 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg
      781 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa
      841 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat
      901 gtacttggaa tttcagta
//
