LOCUS       sequence-136743-         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of sgRNA-15xPBSb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1375-pX-sgRNA-15xPBSb
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
       61 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      121 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      181 aaaggacgaa acaccgggtc ttcgagaaga cctgtttaag agctatgctg gaaacagcat
      241 agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgcc
      301 aattgggtct ccagatttga tatagccttg atatagcctt gatatagcct tgatatagcc
      361 ttgatataag atttgatata gccttgatat agccttgata tagccttgat atagccttga
      421 tataagattt gatatagcct tgatatagcc ttgatatagc cttgatatag ccttgatata
      481 agatcttttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgtagcgc
      541 gtgcgccaat tctgcagaca aatggcggcg cgccgcggcc gcaggaaccc ctagtgatgg
      601 agttggccac tccctctctg cgcgct
//
