LOCUS       sequence-136747-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of sgRNA-20xPBSc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1399-pX-sgRNA-20xPBSc
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
       61 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      121 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      181 gaaaggacga aacaccgggt cttcgagaag acctgtttaa gagctatgct ggaaacagca
      241 tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 caattgggtc tccagatttg atgtagcctt gatgtagcct tgatgtagcc ttgatgtagc
      361 cttgatgtaa gatttgatgt agccttgatg tagccttgat gtagccttga tgtagccttg
      421 atgtaagatt tgatgtagcc ttgatgtagc cttgatgtag ccttgatgta gccttgatgt
      481 aagatttgat gtagccttga tgtagccttg atgtagcctt gatgtagcct tgatgtaaga
      541 tctttttttg ttttagagct agaaatagca agttaaaata aggctagtcc gtagcgcgtg
      601 cgccaattct gca
//
