LOCUS       sequence-136756-         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of sgRNA-15xPBSa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1372-pX-sgRNA-15xPBSa
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc gggtcttcga gaagacctgt ttaagagcta tgctggaaac
      241 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      301 gtgccaattg ggtctccaga ttgtatgtag cctgtatgta gcctgtatgt agcctgtatg
      361 tagcctgtat gtaagattgt atgtagctgt atgtagcctg tatgtagcct gtatgtagcc
      421 tgtatgtaag attgtatgta gcctgtatgt agcctgtatg tagcctgtat gtagcctgta
      481 tgtaagatct ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgta
      541 gcgcgtgcgc caattctgca gacaaatggc ggcgcgccgc ggccgcagga acccctagtg
      601 atggagttgg ccactccctc tctgcgcgc
//
