LOCUS       sequence-136763-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  intronically embedded eSIBR backbone in gateway middle entry vector 
            (pDONR221).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Fowler DK, Williams C, Gerritsen AT, Washbourne P
  TITLE     Improved knockdown from artificial microRNAs in an enhanced miR-155 
            backbone: a designer's guide to potent multi-target RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Nov 17. pii: gkv1246.
  PUBMED    26582923
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 17. pii: gkv1246."
COMMENT     Sequence Label: pME-eSIBR
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagctaa
      121 ggcgcgcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
      181 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
      241 agatgtccct tgtatcacca tgcatggacc ctcatgataa ttttgtttct ttcactttct
      301 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
      361 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
      421 taagatccca tcgattcgaa ttcaaagtcg actggaggct tgctttgggc tgtatgctgt
      481 tgtcttcaag atctggaaga cacaggacac aaggcccttt atcagcactc acatggaaca
      541 aatggccacc gtgggaggat gacaactcga gcctctagac aatcagggta tattatattg
      601 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
      661 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
      721 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
      781 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
      841 acagctcctg ggcaacgtgc tgacccagct ttcttg
//
