LOCUS       sequence-136764-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for expression of sgRNA-25xPBSb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1376-pX-sgRNA-25xPBSb
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..143
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
       61 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      121 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      181 ttgtggaaag gacgaaacac cgggtcttcg agaagacctg tttaagagct atgctggaaa
      241 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      301 ggtgccaatt gggtctccag atttgatata gccttgatat agccttgata tagccttgat
      361 atagccttga tataagattt gatatagcct tgatatagcc ttgatatagc cttgatatag
      421 ccttgatata agatttgata tagccttgat atagccttga tatagccttg atatagcctt
      481 gatataagat ttgatatagc cttgatatag ccttgatata gccttgatat agccttgata
      541 taagatttga tatagccttg atatagcctt gatatagcct tgatatagcc ttgatataag
      601 atcttttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtagcgcgt
      661 gcgccaattc tgcagacaaa tggcggcgcg ccgcggccgc aggaacccct agtgatggag
      721 ttggccactc cctctctgcg cgc
//
