LOCUS       sequence-136769-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP21
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg
       61 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc
      121 aaacaagttt gtacaaaaaa gttggcatgc cccaggcctc tgagcaccgc ctgggccgta
      181 cccgagagcc acctgttaat atccagcccc gagtgggatc caagctacca tttgccccca
      241 gggcccgcag caaggagcgc agaaacccag gctctgggcc aaaccccatg ttacgacctc
      301 tgcctccccg gccaggtctg cctgatgaac ggctcaagaa actggagctg ggacggggac
      361 ggacctcagg ccctcgtccc agaggccccc ttcgagcaga tcatggggtt cccctgcctg
      421 gctcaccacc cccaacagtg gctttgcctc tcccatctcg gaccaactta gcccgttcca
      481 agtctgtgag cagtggggac ttgcgtccaa tggggattgc cttgggaggg caccgtggca
      541 ccggagagct tggggctgca ctgagccgct tggccctccg gcctgagcca cccactttga
      601 gacgtagcac ttctctccgc cgcctagggg gctttcctgg accccctacc ctgttcagca
      661 tacggacaga gccccctgct tcccatggct ccttccacat gatatccgcc cggtcctctg
      721 agcctttcta ctctgatgac aagatggctc atcacacact ccttctgggc tctggtcatg
      781 ttggccttcg aaacctggga aacacgtgct tcctgaatgc tgtgctgcag tgtctga
//
