LOCUS       sequence-136771-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFb_VP64 in expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1356-pmax-NLSPUFb_VP64
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          83..215
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    230..254
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             293..313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat tgctaacgca
       61 gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt aaggaggcca
      121 atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg cacctattgg
      181 tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga caagtttgta
      241 caaaaaagca ggctccgaat tcaccggtgc cgccaccatg gggatcctcc cccccaagaa
      301 aaagaggaag gtatctagag gccgcagccg ccttttggaa gattttcgaa acaaccggta
      361 ccccaattta caactgcggg agattgctgg acatataatg gaattttccc aagaccagca
      421 tgggtccaga ttcattcagc tgaaactgga gcgtgccaca ccagctgagc gccagcttgt
      481 cttcaatgaa atcctccagg ctgcctacca actcatggtg gatgtgtttg gtaattacgt
      541 cattcagaag ttctttgaat ttggcagtct tgaacagaag ctggctttgg cagaacggat
      601 tcgaggccac gtcctgtcat tggcactaca gatgtatggc tgccgtgtta tccagaaagc
      661 tcttgagttt attccttcag accagcagaa tgagatggtt cgggaactag atggccatgt
      721 cttgaagtgt gtgaaagatc agaatggcaa tcacgtggtt cagaaatgca ttgaatgtgt
      781 acagccccag tctttgcaat ttatcatcga tgcgtttaag ggacaggtat ttgccttatc
      841 cacacatcct tatggctgcc gagtgattca
//
