LOCUS       sequence-136777-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ratiometric pHluorin2 with a gpi linkage sequence in a middle entry 
            clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Stawicki TM, Owens KN, Linbo T, Reinhart KE, Rubel EW, Raible DW
  TITLE     The zebrafish merovingian mutant reveals a role for pH regulation in
            hair cell toxicity and function.
  JOURNAL   Dis Model Mech. 2014 Jul;7(7):847-56. doi: 10.1242/dmm.016576.
  PUBMED    24973752
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dmm"; 
            journalName: "Dis Model Mech"; date: "2014-07"; volume: "7"; issue: 
            "7"; pages: "847-56"
COMMENT     Sequence Label: pME phluorin2-gpi
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(127..148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(388..407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     735..756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggctcagc ggatgacaac acagctgctg ctccttctag tgtgggtggc tgtagtaggg
       61 gaggctcaga caaggattgc aatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accctgagct acggcgtgca gtgcttcagc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acgagcacct ggtgtacatc
      541 atggccgaca agcagaagaa cggcaccaag gccatcttcc aggtgcacca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgcac acccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc
      781 atggacgagc tgtacaaggc tgcagccatg agtggggctg ggccctgggc agcctggcct
      841 ttcctgctta gcctggccct aatgctgctg tggctgctca gctga
//
