LOCUS       sequence-136821-        2184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai1-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1.2-Orai1
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             814..1254
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     regulatory      1577..1586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct cactcgagaa cgtctatatc aaggccgacg agcagaagaa cggcatcaag
      121 gcgtacttca agatccgcca caacatcgag ggcggcggcg tgcagctcgc ctaccactac
      181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 gtgcagtcca tgctttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
      361 gggagcgaat ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatccag
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag
      661 cgcaccatct tcttcaaggg cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaacacg cgtgaccaac tgactgaaga gcagatcgca
      841 gaatttaaag aggctttctc cctatttgac aaggacgggg atgggacgat aacaaccaag
      901 gagctgggga cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac
      961 atgatcaatg aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca
     1021 atgatggcaa gaaaaatgaa agacacagac agtgaagaag aaattagaga agcgttccgt
     1081 gtgtttgata aggacggcaa tggctacatc ggcgcagcag agcttcgcca cgtgatgaca
     1141 aaccttggag agaagttaac agatgaagag gttgatgaaa tgatcagggt agcagacatc
     1201 gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagcagatc
     1261 tcgagctcaa gcttcgaatt ccatccggag cccgccccgc ccccgagccg cagcagtccc
     1321 gagcttcccc caagcggcgg cagcaccacc agcggcagcc gccggagccg ccgccgcagc
     1381 ggggacgggg agcccccggg ggccccgcca ccgccgccgt ccgccgtcac ctacccggac
     1441 tggatcggcc agagttactc cgaggtgatg agcctcaacg agcactccat gcaggcgctg
     1501 tcctggcgca agctctactt gagccgcgcc aagcttaaag cctccagccg gacctcggct
     1561 ctgctctccg gcttcgccat ggtggcaatg gtggaggtgc agctggacgc tgaccacgac
     1621 tacccaccgg ggctgctcat cgccttcagt gcctgcacca cagtgctggt ggctgtgcac
     1681 ctgtttgcgc tcatgatcag cacctgcatc ctgcccaaca tcgaggcggt gagcaacgtg
     1741 cacaatctca actcggtcaa ggagtccccc catgagcgca tgcaccgcca catcgagctg
     1801 gcctgggcct tctccaccgt catcggcacg ctgctcttcc tagctgaggt ggtgctgctc
     1861 tgctgggtca agttcttgcc cctcaagaag cagccaggcc agccaaggcc caccagcaag
     1921 ccccccgcca gtggcgcagc agccaacgtc agcaccagcg gcatcacccc gggccaggca
     1981 gctgccatcg cctcgaccac catcatggtg cccttcggcc tgatctttat cgtcttcgcc
     2041 gtccacttct accgctcact ggttagccat aagaccgacc gacagttcca ggagctcaac
     2101 gagctggcgg agtttgcccg cttacaggac cagctggacc acagagggga ccaccccctg
     2161 acgcccggca gccactatgc ctag
//
