LOCUS       sequence-136824-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-2A-MCS2
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..175)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(216..530)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(219..243)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    290..308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    326..344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    361..379
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    397..415
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    433..451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    468..486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    504..522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    584..701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(914..958)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
       61 gctgccttgg aaaaggcgca accccaaccc cggatcctgt acaacgcgtc tgcagcctag
      121 gaccggggtt ttcttccacg tctcctgctt gctttaacag agagaagttc gtggctccgg
      181 aaccggtgtc gacgaattcg ctagccaatt ctccaggcga tctgacggtt cactaaacga
      241 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacatacgtt ctctatcact
      301 gatagggagt aaactcgaca tacgttctct atcactgata gggataaact cgacatacgt
      361 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
      421 ctcgacatac gttctctatc actgataggg agtaaactcg acatcgttct ctatcactga
      481 tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc gacatatcga
      541 ttcgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      601 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      661 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      721 tttgtctcga ggtcgagaat tgtcccctcg gggttgggag gtgggtctga aacgataatg
      781 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
      841 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
      901 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
      961 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     1021 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     1081 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
//
