LOCUS       sequence-136832-         504 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression with FACS-selectable marker (extracellular 
            part of NGFR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  AUTHORS   van_de_Vosse E, Verhard EM, de Paus RA, Platenburg GJ, van Deutekom 
            JC, Aartsma-Rus A, van Dissel JT
  TITLE     Antisense-mediated exon skipping to correct IL-12Rbeta1 deficiency 
            in T cells.
  JOURNAL   Blood. 2009 May 7;113(19):4548-55. doi: 
            10.1182/blood-2008-12-196220. Epub 2009 Mar 3.
  PUBMED    19258592
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2008-12-196220"; journalName: "Blood"; date: 
            "2009-05-7- 7"; volume: "113"; issue: "19"; pages: "4548-55"
COMMENT     Sequence Label: pLZRS-IRES-deltaNGFR
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    49..423
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     361..377
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     457..473
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     458..474
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
       61 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
      121 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
      181 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
      241 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
      301 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
      361 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
      421 ctgccggatc catttaaatt cgaattcctg caggcctcga ggtcgacggt atcgataagc
      481 ttgatatcga attaattccg cccc
//
