LOCUS       sequence-136847-        1521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for selection for insertion and homodimerization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Elazar A, Weinstein J, Biran I, Fridman Y, Bibi E, Fleishman SJ
  TITLE     Mutational scanning reveals the determinants of protein insertion 
            and association energetics in the plasma membrane.
  JOURNAL   Elife. 2016 Jan 29;5. pii: e12125. doi: 10.7554/eLife.12125.
  PUBMED    26824389
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-01-29"; volume: "5. pii: e12125. doi"; pages: " 
            10.7554/eLife.12125"
COMMENT     Sequence Label: pMAL_dst-beta-L
FEATURES             Location/Qualifiers
     source          1..1521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             499..1287
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(645..664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaggtgattt ctcgcaatga tttgcatgac tttgtttggc gagagcaagg ttttgaagtc
       61 gatgattcca gcttaaccca agccatttcg actctgcgca aaatgctcaa agattcgaca
      121 aagtccccac aatacgtcaa aacggttccg aagcgcggtt accaattgat cgcccgagtg
      181 gaaacggttg aagaagagat ggctcgcgaa aacgaagctg ctcatgacat ctctcagcca
      241 gaatctgtca atgaatacgc agaatcaagc agtgtgcctt catcagccac tgtagtgaac
      301 acaccgcagc cagccaatgt cgtggcgaat aaatcggctc caaacttggg gaatcgactc
      361 gagccgctgt tcatcccggt tgcagttatg gttaccgctt ttagtggatt ggcgtttatc
      421 atctggctgg ctactagtgg ggggtctgat ccaagccaat ccagctttaa acccctaacg
      481 cagttaaccg gtggtcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      661 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      961 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1261 ggtgcctcac tgattaagca ttggtaggta cccggccggg gtctagcatg ttataataac
     1321 tataactaat aacgtaacgt gactggcaag agatattttt aaaacaatga ataggtttac
     1381 acttacttta gttttatgga aatgaaagat catatcatat ataatctagc tagataaaaa
     1441 atttagaagc caatgaaatc tataaataaa ctaaattaag tttatttaat taacaactat
     1501 ggatataaaa taggtactaa t
//
