LOCUS       sequence-136850-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Peredox fluorescent sensor protein (for NADH/NAD 
            ratio) in mammalian cells (and can be packaged as AAV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Mongeon R, Venkatachalam V, Yellen G
  TITLE     Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon 
            Fluorescence Lifetime Biosensor Imaging.
  JOURNAL   Antioxid Redox Signal. 2016 Mar 18.
  PUBMED    26857245
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2016 Mar 18."
COMMENT     Sequence Label: pAAV-CAG-Peredox
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2281..2300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2459..2588
                     /label=AAV2 ITR
     promoter        2899..3176
                     /label=chicken beta-actin promoter
     primer_bind     3456..3478
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3540..3559
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3594..3627
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(5732..5765)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5807..6395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5860..5880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6278..6289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6438..6559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6503..6522
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6721..6850
                     /label=AAV2 ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagagtacc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2881 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2941 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     3001 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     3061 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     3121 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     3181 tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     3241 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     3301 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     3361 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcggggct gtccgcgggg
     3421 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     3481 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     3541 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc cccataactt
     3601 cgtataaagt atcctatacg aagttatact agaattcggt accaccggtg ctagattggg
     3661 cccaccatgg ttgctagcaa agttcctgaa gcagccattt ccagactgat tacttacctg
     3721 agaattctgg aagagctgga agcccaaggt gtacaccgca ccgcctccga gcaactgggc
     3781 gagctggccc aggtcaccgc cttccaggtt gataaggacc tgtcctactt cggctcctac
     3841 ggcactgacg gcgttggcta cactgtaccg gtcctcaaga gagaactgcg ccatatcctc
     3901 ggtctgaacc gcaaatgggg cctgtgcatc gtggggatgg gccgcctggg atccgctctt
     3961 gctgattggc ctggttttgg cgagagcttc gagctgcgcg gtttcttcga tgttgaccca
     4021 ggtatggttg gtcggccggt tcgcggtggt gttatcgaac acgttgatct gctgccccag
     4081 cgcgtacctg gtcgcatcga aatcgctctg ctgacggttc cacgcgaggc agcacagaag
     4141 gctgccgacc tgctggttgc cgctggcatc aagggcatcc tgaacttcgc tccagttgta
     4201 ctggaggttc ccaaggaagt ggctgttgag aacgttgaca tcctggccgg tctgacgcgt
     4261 ctgagctttg ccattctgaa ccccacgtgg agcgcagcag gtgggcatgg tttcactgct
     4321 cacaacgtgt atatcatggc tgacaaacag aagaacggta tcaaggccaa cttcaagatc
     4381 cgccacaaca tcgaagacgg cggcgttcag ctggctgatc actaccagca gaacacccca
     4441 atcggcgacg gccctgttct gctgcctgac aaccactact taagcatcca gtctaaactg
     4501 tccaaagacc caaacgagaa gcgcgaccac atggttctgc tggaatttgt taccgccgct
     4561 ggcatcaccc acggcatgga cgaactgtac aagggcggca ccgggggatc tatggtttcc
     4621 aaaggcgagg aactgttcac tggtgttgtt cctatcctgg ttgaactgga cggcgacgtt
     4681 aacggccaca aattcagcgt tagcggggaa ggcgaaggag acgctaccta cggcaagctg
     4741 actctgaagt tcatctgcac cactggcaag ctgcctgttc cttggcctac cctggttact
     4801 accttctcct atggtgttat ggttttcgcc agatatcctg accacatgaa gcagcacgac
     4861 ttcttcaaga gcgccatgcc tgagggctac gttcaggaac gcaccatctt tttcaaggac
     4921 gacggcaact ataagacacg cgctgaggtt aagttcgagg gcgacactct ggttaaccgc
     4981 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctgggcca taagcttgaa
     5041 tataacacca aggtacctga agccgccatc agccgcctca tcacttacct ccgcatcctg
     5101 gaggagctcg aagcccaagg cgttcaccgc actgcaagcg agcagctggg cgaactggca
     5161 caggttacag cattccaggt tgacgaagat ctgtcctatt tcggttccta cggcaccgac
     5221 ggtgttggct acacagtccc tgtcctgaag cgcgagctgc gccacatcct gggcctgaac
     5281 cgcaagtggg gcttatgcat cgttggcatg ggccgcctcg gtagtgcact ggcagactgg
     5341 ccgggctttg gtgaatcttt cgaactgcgc ggcttcttcg acgttgatcc aggcatggtt
     5401 gggcgccctg ttcgcggtgg cgttatcgag catgttgacc tcctgccaca gcgcgtcccg
     5461 ggtcgcatcg agatcgccct gctgaccgtt cctcgcgaag ctgcacagaa ggcagccgac
     5521 ttactagtcg ccgctggtat caagggaatt ctgaacttcg cccctgttgt tctggaggtt
     5581 cctaaggaag ttgctgttga aaacgttgat ttcctggccg gcctgacccg cctgagcttc
     5641 gcaatcctga atcctaagtg gcgcgaggaa atgatgggtt gactcgacga attcattgat
     5701 cctcgagtgt acaagtaagg ggatccctag tataacttcg tatagcatac attatacgaa
     5761 gttatgtccc tcgaagaggt tcgaattcga tatcaagctt atcgataatc aacctctgga
     5821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6181 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6361 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc
     6421 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6481 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6541 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6601 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     6661 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
     6721 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     6781 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     6841 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6901 gcgct
//
