LOCUS       sequence-136851-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFw_VP64 in expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1357-pmax-NLSPUFw_VP64
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          85..217
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      274..283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             295..315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgaaccgtca gatcactagt agctttattg cggtagttta tcacagttaa attgctaacg
       61 cagtcagtgc tcgactgatc acaggtaagt atcaaggtta caagacaggt ttaaggaggc
      121 caatagaaac tgggcttgtc gagacagaga agattcttgc gtttctgata ggcacctatt
      181 ggtcttactg acatccactt tgcctttctc tccacaggga aaaaacaatt gacaagtttg
      241 tacaaaaaag caggctccga attcaccggt gccgccacca tggggatcct cccccccaag
      301 aaaaagagga aggtatctag aggccgcagc cgccttttgg aagattttcg aaacaaccgg
      361 taccccaatt tacaactgcg ggagattgct ggacatataa tggaattttc ccaagaccag
      421 catgggtcca gattcattca gctgaaactg gagcgtgcca caccagctga gcgccagctt
      481 gtcttcaatg aaatcctcca ggctgcctac caactcatgg tggatgtgtt tggtaattac
      541 gtcattcaga agttctttga atttggcagt cttgaacaga agctggcttt ggcagaacgg
      601 attcgaggcc acgtcctgtc attggcacta cagatgtatg gctgccgtgt tatccagaaa
      661 gctcttgagt ttattccttc agaccagcag aatgagatgg ttcgggaact agatggccat
      721 gtcttgaagt gtgtgaaaga tcagaatggc aatcacgtgg ttca
//
