LOCUS       sequence-136852-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFc_p65HSF1 in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1411-pmax-NLSPUFc_p65HSF1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgtacaaa aaagcaggct ccgaattcac cggtgccgcc accatgggga tcctcccccc
       61 caagaaaaag aggaaggtat ctagaggccg cagccgcctt ttggaagatt ttcgaaacaa
      121 ccggtacccc aatttacaac tgcgggagat tgctggacat ataatggaat tttcccaaga
      181 ccagcatggg tccagattca ttcagctgaa actggagcgt gccacaccag ctgagcgcca
      241 gcttgtcttc aatgaaatcc tccaggctgc ctaccaactc atggtggatg tgtttggtaa
      301 ttacgtcatt cagaagttct ttgaatttgg cagtcttgaa cagaagctgg ctttggcaga
      361 acggattcga ggccacgtcc tgtcattggc actacagatg tatggcagcc gtgttatcga
      421 gaaagctctt gagtttattc cttcagacca gcagaatgag atggttcggg aactagatgg
      481 ccatgtcttg aagtgtgtga aagatcagaa tggcaatcac gtggttcaga aatgcattga
      541 atgtgtacag ccccagtctt tgcaatttat catcgacgcg tttaagggac aggtatttgc
      601 cttatccaca catccttatg gctgccgagt gattcagaga atcctggagc actgtctccc
      661 tgaccagaca ctccctattt tagaggagct tcaccagcac acagagcagc tggtacagga
      721 tcaatatgga agttatgtaa tcgaacatgt actggagcac ggtcgtcctg aggataaaag
      781 caaaattgta gcagaaatcc gaggcaatgt acttgtattg agtcagcaca aatttgcaaa
      841 caatgttgtg cagaagtgtg ttactcacgc ctcacgtacg gagcgcgctg tgctcatc
//
