LOCUS       sequence-136854-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4xNLS_PUFa_2xNLS_mCBPHAT in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1417-pmax-4xNLS_PUFa_2xNLS_mCBPHAT
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          96..228
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    243..267
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             318..338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             342..362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             366..386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             477..497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aggtcgttta gtgaaccgtc agatcactag tagctttatt gcggtagttt atcacagtta
       61 aattgctaac gcagtcagtg ctcgactgat cacaggtaag tatcaaggtt acaagacagg
      121 tttaaggagg ccaatagaaa ctgggcttgt cgagacagag aagattcttg cgtttctgat
      181 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggg aaaaaacaat
      241 tgacaagttt gtacaaaaaa gcaggctccg aattcaccgg tgccgccacc atgatcgatg
      301 gcggaggcgg atctgatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg
      361 tagatccaaa aaagaagaga aaggtaggat ctaccggatc tagaaacgat ggtggtggtg
      421 gaagcggggg tgggggcagc ggtggagggg gaagcgggcg cgccgggatc ctccccccca
      481 agaaaaagag gaaggtatct agaggccgca gccgcctttt ggaagatttt cgaaacaacc
      541 ggtaccccaa tttacaactg cgggagattg ctggacatat aatggaattt tcccaagacc
      601 agcatgggtc cagattcatt cagctgaaac tggagcgtgc cacaccagct gagcgccagc
      661 ttgtcttcaa tgaaatcctc caggctgcct accaactcat ggtggatgtg tttggtaatt
      721 acgtcattca gaagttcttt gaatttggca gtcttgaaca gaagctggct ttggcagaac
      781 ggattcgagg ccacgtcctg tcattggcac tacagatgta tggcagccgt gttatcgaga
      841 aagctcttga gtttattcct tcagaccagc agaatgagat ggttcgggaa ctagatggcc
      901 atgtcttgaa gtgtgtgaaa
//
