LOCUS       sequence-136855-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFa_VP64 in transient expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1355-pmax-NLSPUFa_VP64
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          95..227
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    242..266
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             305..325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtcgtttag tgaaccgtca gatcactagt agctttattg cggtagttta tcacagttaa
       61 attgctaacg cagtcagtgc tcgactgatc acaggtaagt atcaaggtta caagacaggt
      121 ttaaggaggc caatagaaac tgggcttgtc gagacagaga agattcttgc gtttctgata
      181 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggga aaaaacaatt
      241 gacaagtttg tacaaaaaag caggctccga attcaccggt gccgccacca tggggatcct
      301 cccccccaag aaaaagagga aggtatctag aggccgcagc cgccttttgg aagattttcg
      361 aaacaaccgg taccccaatt tacaactgcg ggagattgct ggacatataa tggaattttc
      421 ccaagaccag catgggtcca gattcattca gctgaaactg gagcgtgcca caccagctga
      481 gcgccagctt gtcttcaatg aaatcctcca ggctgcctac caactcatgg tggatgtgtt
      541 tggtaattac gtcattcaga agttctttga atttggcagt cttgaacaga agctggcttt
      601 ggcagaacgg attcgaggcc acgtcctgtc attggcacta cagatgtatg gcagccgtgt
      661 tatcgagaaa gctcttgagt ttattccttc agaccagcag aatgagatgg ttcgggaact
      721 agatggccat gtcttgaagt gtgtgaaaga tcagaatggc aatcacgtgg ttcagaaatg
      781 cattgaatgt gtacagcccc agtctttgca atttatcatc gatgcgttta agggacaggt
      841 atttgcctta tccacacatc cttatggctg ccgagtgatt cagagaatcc tggagcactg
      901 tctccctgac cagacactcc ctattttaga ggagcttcac c
//
