LOCUS       sequence-136869-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCBPHAT_4xNLS_PUFa_2xNLS in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1418-pmax-mCBPHAT_4xNLS_PUFa_2xNLS
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          94..226
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    241..265
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtcgtttagt gaaccgtcag atcactagta gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcagtgct cgactgatca caggtaagta tcaaggttac aagacaggtt
      121 taaggaggcc aatagaaact gggcttgtcg agacagagaa gattcttgcg tttctgatag
      181 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa aaaacaattg
      241 acaagtttgt acaaaaaagc aggctccgaa ttcaccggtg ccgccaccat gatctttaaa
      301 cccgaggagc tacgccaggc acttatgcca actctagaag cactctatcg acaggaccca
      361 gagtctttgc cttttcgtca gcctgtagat cctcagctcc taggaatccc agattatttt
      421 gatatagtga agaatcctat ggacctttct accatcaaac gaaagctgga cacagggcaa
      481 tatcaagaac cctggcagta tgtggatgat gtctggctta tgttcaacaa tgcgtggcta
      541 tataatcgta aaacgtcccg tgtatataaa ttttgcagta aacttgcaga ggtctttgaa
      601 caagaaattg accctgtcat gcagtctctt ggatattgct gtggacgaaa gtatgagttc
      661 tccccacaga ctttgtgctg ttacggaaag cagctgtgta caattcctcg tgatgcagcc
      721 tactacagct atcagaatag gtatcatttc tgtgagaagt gtttcacaga gatccagggc
      781 gagaatgtga ccctgggtga cgacccttcc caacctcaga cgacaatttc caaggatcaa
      841 tttgaaaaga agaaaaatga taccttagat cctgaacctt ttgttgactg caaagagtgt
      901 ggccggaaga tgc
//
