LOCUS       sequence-136877-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9-3xGGGGS-VP64 in pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC164-pmax-dCas9Master-VP64
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..26
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacaagtttg tacaaaaaag caggctccga attcaccggt gccgccacca tgatcgatgg
       61 tggcggtggt agcgggggag gcggctccgg gggcggaggc agtatgtacc catacgatgt
      121 tccagattac gcttcgccga agaaaaagcg caaggtcgaa gcgtccgaca agaagtacag
      181 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      241 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      301 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      361 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      421 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      481 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
      541 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
      601 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
      661 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
      721 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
      781 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
      841 cgccca
//
