LOCUS       sequence-136878-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of a modified version of the fission yeast NatA 
            complex - chloramphenicol marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP
  TITLE     An enhanced recombinant amino-terminal acetylation system and novel 
            in vivo high-throughput screen for molecules affecting 
            alpha-synuclein oligomerisation.
  JOURNAL   FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.
  PUBMED    28214355
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2017 Feb 18. doi: 10.1002/1873-3468.12597."
COMMENT     Sequence Label: pNatA (pACYCduet-naa10-naa15)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    635..659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctggcgttc aaatttcgca gcagcggttt ctttaccaga ctcgagggta ccagatcttc
       61 aattgttcaa tgctaaactt tgtaccgtct ttgccagttc atcatttgtc tccggagtat
      121 cgaatttcag cgttgaaaag tctttatgca tcgcatacgc gtcctccccg tcggcataat
      181 acttggattc aatgccctga acgtcaaact gcagggtatc acggtataaa tggatcgcgg
      241 cacgattgct cttccgcaca tgcagggaca tatatttggc gccatacact tcgaccatcg
      301 cgcgctggct ttgaaccatc aggcgtttgg cgagacctaa atggcggtaa ctacgcatca
      361 cggaaacgct agtgatatgg ccatgcggaa taccatcctt tggctcttct tccatttttg
      421 caaggacata gccaacaaca cgacctttcg ggtcagttgc aacatagctc agcataggcc
      481 agctgatcgc gtgataaaga taatatttca gttggtaatt ttcgggcaga ttgtgcaggt
      541 tgcaattctg cataccagtc aggtcactaa tgcgcgcggg acgaatatcc atatgtatat
      601 ctccttctta tacttaacta atatactaag atggggaatt gttatccgct cacaattccc
      661 ctatagtgag tcgtattaat ttcgattatg cggccgtgta caatacgatt actttctgtt
      721 cgacttaagc attatgcggc cgcttagtta gactgagtt
//
