LOCUS       sequence-136880-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLSPUFc_VP64 in expression vector pmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1358-pmax-NLSPUFc_VP64
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             66..86
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgacaagttt gtacaaaaaa gcaggctccg aattcaccgg tgccgccacc atggggatcc
       61 tcccccccaa gaaaaagagg aaggtatcta gaggccgcag ccgccttttg gaagattttc
      121 gaaacaaccg gtaccccaat ttacaactgc gggagattgc tggacatata atggaatttt
      181 cccaagacca gcatgggtcc agattcattc agctgaaact ggagcgtgcc acaccagctg
      241 agcgccagct tgtcttcaat gaaatcctcc aggctgccta ccaactcatg gtggatgtgt
      301 ttggtaatta cgtcattcag aagttctttg aatttggcag tcttgaacag aagctggctt
      361 tggcagaacg gattcgaggc cacgtcctgt cattggcact acagatgtat ggcagccgtg
      421 ttatcgagaa agctcttgag tttattcctt cagaccagca gaatgagatg gttcgggaac
      481 tagatggcca tgtcttgaag tgtgtgaaag atcagaatgg caatcacgtg gttcagaaat
      541 gcattgaatg tgtacagccc cagtctttgc aatttatcat cgacgcgttt aagggacagg
      601 tatttgcctt atccacacat ccttatggct gccgagtgat tcagagaatc ctggagcact
      661 gtctccctga ccagacactc cctattttag aggagcttca ccagcacaca gagcagctgg
      721 tacaggatca atatggaagt tatgtaatcg aacatgtact ggagcacggt cgtcctgagg
      781 ataaaagcaa aattgtagca gaaatccgag gcaatgtact tgtattgagt cagcacaaat
      841 ttgcaaacaa tgttgtgcag aagtgtgtta ctcacgcctc acgtacggag cgcgctgtgc
      901 tcatcgacga ggtgtgcacc atgaacgacg gt
//
