LOCUS       sequence-136884-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter panel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-p15A-J23113-deGFP-T500 (124p)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        226..260
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     RBS             273..295
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(332..353)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(593..612)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacgaccttt ctgtggtgaa accggatgct gcaattcaga gcgccagcaa gtgggggaca
       61 gcagaagacc tgaccgccgc agagtggatg tttgacatgg tgaagactat cgcaccatca
      121 gccagaaaac cgaattttgc tgggtgggct aacgatatcc gcctgatgcg tgaacgtgac
      181 ggacgtaacc accgcgacat gtgtgtgctg ttccgctggg catgcctgat ggctagctca
      241 gtcctaggga ttatgctagc gctagcaata attttgttta actttaagaa ggagatatac
      301 catggagctt ttcactggcg ttgttcccat cctggtcgag ctggacggcg acgtaaacgg
      361 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      421 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      481 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      541 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      601 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      661 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      721 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      781 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      841 gaacacc
//
